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A Horseradish Peroxidase (HRP) conjugated detection antibody is then added and incubate
Then a biotinylated detection antibody specific for Mouse HSP-70/HSPA9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
And then biotinylated Rat MDH1 Antibody is added and binds to MDH1 in the sample
UBIAD1 present in the sample is added and binds to antibodies coated on the wells
And then biotinylated Human FLG Antibody is added and binds to FLG in the sample
Human IL-25 (Interleukin 25) ELISA Kit Packaging:96 wells A Horseradish Peroxidase (HRP) conjugatedThe Human (IL 25) Interleukin 25 ELISA Kit measures Interleukin 25 in samples. The plate has been pre coated with Human IL25 antibody. IL25 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IL25 Antibody is added and binds to IL25 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IL25 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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