Rat SYNPO (Synaptopodin) ELISA Kit Packaging:96 wells Substrate solution is then added

Substrate solution is then added and color develops in proportion to the amount of Human ALMS1

Substrate solution is then added and color develops in proportion to the amount of Human CST4

And then biotinylated Mouse EDIL3 Antibody is added and binds to EDIL3 in the sample

And then biotinylated Rat PGC Antibody is added and binds to PGC in the sample

The concentration of Rat GPX3 in the samples is then determined by comparing the OD of the samples to the standard curve

Rat SYNPO (Synaptopodin) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Rat (SYNPO) Synaptopodin ELISA Kit measures Synaptopodin in Rat samples. The plate has been pre coated with Rat SYNPO antibody. SYNPO present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat SYNPO Antibody is added and binds to SYNPO in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SYNPO antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.