Human AZU (Azurocidin) ELISA Kit Packaging:96 wells Standards or samples are added

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TNFSF13

Substrate solution is then added and color develops in proportion to the amount of Human ATN1

And then biotinylated Human ANXA4 Antibody is added and binds to ANXA4 in the sample

The microtiter plate is coated with the antibody specifically binding Insulin-like Growth Factor-binding Protein 7

And then biotinylated Canine KRT10 Antibody is added and binds to KRT10 in the sample

Human AZU (Azurocidin) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human (AZU) Azurocidin ELISA Kit measures Azurocidin in samples. The plate has been pre coated with Human AZU1 antibody. AZU1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human AZU1 Antibody is added and binds to AZU1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated AZU1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate