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The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human αFP
the generation buffer is added to bring the pH of the sample to approximately 6
The assay plate has been pre-coated with mouse anti-Mouse CD5L monoclonal antibody
IGFL4 present in the sample is added and binds to antibodies coated on the wells
Then a biotinylated detection antibody specific for Rat SHBG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human IL-24 (Interleukin 24) ELISA Kit Packaging:96 wells The micro ELISA plate providedThe Human (IL 24) Interleukin 24 ELISA Kit measures Interleukin 24 in samples. The plate has been pre coated with Human IL 24 antibody. IL 24 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IL 24 Antibody is added and binds to IL 24 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IL 24 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
