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The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for IGFBP-1 is immobilized onto the microplate and another monoclonal antibody specific for a different epitope of IGFBP-1 is conjugated to horse radish peroxidase (HRP conjugate)
only those wells that contain Human IL3
and the color intensity is directly proportional to the amount of Human RANKL present
The plate has been pre-coated with Mouse FCGRT antibody
BTN1A1 present in the sample is added and binds to antibodies coated on the wells
Rat HSP90AB1 (Heat Shock Protein Hsp 90-beta) ELISA Kit Packaging:96 wells The assay makes use ofThe Rat (HSP90AB1) Heat Shock Protein Hsp 90 beta ELISA Kit measures Heat Shock Protein Hsp 90 Beta in Rat samples. The plate has been pre coated with Rat HSP90AB1 antibody. HSP90AB1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat HSP90AB1 Antibody is added and binds to HSP90AB1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated HSP90AB1 antibody. After incubation unbound
