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Substrate solution is then added and color develops in proportion to the amount of Human SYT3
it binds with UGT1A6 bound to precoated antibody
Then Streptavidin-HRP is added and binds to the Biotinylated AGRP antibody
You can calculate the concentration of Mouse gp130 in the samples by comparing the OD of the samples to the standard curve
The concentration of CD32 in the sample was calculated by drawing a standard curve
Mouse CNTF (Ciliary neurotrophic factor) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Mouse CNTF (Ciliary neurotrophic factor) ELISA Kit measures Cntf in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Cntf antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
