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Calculate the concentration of AS-IgG in the sample by plotting standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated AFTPH antibody
ABCD1 present in the sample is added and binds to antibodies coated on the wells
The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 50 nm
it binds with GPC4 bound to precoated antibody
Rabbit EMMPRIN (CD147) ELISA Kit Packaging:96 wells Calculate the concentration of AS-IgGThe Rabbit EMMPRIN (CD147) ELISA Kit measures Emmprin in Rabbit Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Emmprin antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
