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And then biotinylated Human C7 Antibody is added and binds to C7 in the sample
only those wells that contain Human EREG
Then Streptavidin-HRP is added and binds to the Biotinylated HTRA1 antibody
GPC1 present in the sample is added and binds to antibodies coated on the wells
Substrate solution is then added and color develops in proportion to the amount of Bovine IL-7
Rat TPH2 (Tryptophan 5-hydroxylase 2) ELISA Kit Packaging:96 wells And then biotinylated Human C7The Rat (TPH2) Tryptophan 5 hydroxylase 2 ELISA Kit measures Tryptophan 5 hydroxylase 2 in Rat samples. The plate has been pre coated with Rat TPH2 antibody. TPH2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat TPH2 Antibody is added and binds to TPH2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated TPH2 antibody. After incubation unbound Streptavidin HRP is washed away