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Then a biotinylated detection antibody specific for Rat GAL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
then added a HRP-conjugated antibody specific to Mouse T4 to each microplate well and incubated
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish IL4
biotinylated detection antibody andAvidin-HRP conjugate will appear blue in color
The concentration of Human PLP1 in the samples is then determined by comparing the OD of the samples to the standard curve
Rat CX43 (Connexin 43) ELISA Kit (Small Sample) Lenograsti Then a biotinylated detection antibodyThe Rat CX43 (Connexin 43) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat CX43. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat CX43. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only
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