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Then Streptavidin-HRP is added and binds to the Biotinylated GAST antibody
MLXIPL present in the sample is added and binds to antibodies coated on the wells
Then Streptavidin-HRP is added and binds to the Biotinylated PGAM2 antibody
The OD value is proportional to the concentration of Human FLOT2
Then a biotinylated detection antibody specific for Mouse IgG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human FGF23 (Fibroblast Growth Factor 23) ELISA Kit Packaging:48 wells Then Streptavidin-HRP is added andThe Human FGF23 (Fibroblast Growth Factor 23) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human FGF23. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGF23 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each
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