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Human IL-8 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human IL-8
Then Streptavidin-HRP is added and binds to the Biotinylated SLN antibody
FLRT2 present in the sample is added and binds to antibodies coated on the wells
The plate has been pre-coated with Human ASPH antibody
The plate has been pre-coated with Human DLG4 antibody
Human YWHAE (14-3-3 Protein Epsilon) ELISA Kit Packaging:96 wells Human IL-8 in samples (orThe Human (YWHAE) 14 3 3 Protein Epsilon ELISA Kit measures 14 3 3 Protein Epsilon in samples. The plate has been pre coated with Human YWHAE antibody. YWHAE present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human YWHAE Antibody is added and binds to YWHAE in the sample. Then Streptavidin HRP is added and binds to the Biotinylated YWHAE antibody. After incubation unbound Streptavidin HRP is washed away
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