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GDF8 present in the sample is added and binds to antibodies coated on the wells
And then biotinylated Mouse LBP Antibody is added and binds to LBP in the sample
only those wells that contain Rat CA19-9
And then biotinylated Rat GPLD1 Antibody is added and binds to GPLD1 in the sample
Then a biotinylated detection antibody specific for NGAL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human SDC1 (Syndecan-1, Cd138) ELISA Kit Packaging:96 wells GDF8 present in the sampleThe Human (SDC1) Syndecan 1, Cd138 ELISA Kit measures Syndecan 1, Cd138 in samples. The plate has been pre coated with Human SDC1 antibody. SDC1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SDC1 Antibody is added and binds to SDC1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SDC1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
