Human HTN3 (Histatin-3) ELISA Kit Packaging:96 wells Standards or samples are added

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat eIF4E

Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Sheep E2 are added to the micro ELISA plate wells

Then a biotinylated detection antibody specific for Human AGC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Then Streptavidin-HRP is added and binds to the Biotinylated CBS antibody

The Human THP (Tamm-Horsfall Glycoprotein) ELISA kit uses the Sandwich-ELISA principle

Human HTN3 (Histatin-3) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human (HTN3) Histatin 3 ELISA Kit measures Histatin 3 in samples. The plate has been pre coated with Human HTN3 antibody. HTN3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HTN3 Antibody is added and binds to HTN3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated HTN3 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.