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And then biotinylated Human CUBN Antibody is added and binds to CUBN in the sample
Rat β-EP in the sample or standard competes with a fixed amount of Rat β-EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat β-EP
Calculate the concentration of ESM1 in the sample by plotting standard curve
The concentration of Pig IL6 in the samples is then determined by comparing the OD of the samples to the standard curve
The plate has been pre-coated with Mouse IGFBP3 antibody
Human HMW Adiponectin (High Molecular Weight Adiponectin) ELISA Kit Packaging:96 wells And then biotinylated Human CUBNThe Human HMW Adiponectin (High Molecular Weight Adiponectin) ELISA Kit measures HMW ADP in Human Serum, Plasma, Cell Culture Supernatant. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti HMW ADP antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
