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The plate has been pre-coated with Human AMTN antibody
Substrate solution is then added and color develops in proportion to the amount of Mouse ESAM
Then a biotinylated detection antibody specific for Human VEGFR3/Flt4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Human CDH17 in the samples is then determined by comparing the OD of the samples to the standard curve
BAMBI present in the sample is added and binds to antibodies coated on the wells
Human Anti LH (ANTI-Luteinizing Hormone antibody) ELISA Kit GBP The plate has been pre-coatedThe Human Anti LH (ANTI Luteinizing Hormone antibody) ELISA Kit measures LH Ab in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antigen Sandwich ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP detection
