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The assay plate has been pre-coated with mouse anti-Human IL-12 monoclonal antibody
Then a biotinylated detection antibody specific for Human HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The extracted genomic DNA fragments are large
The concentration of Human CGa in the samples is then determined by comparing the OD of the samples to the standard curve
The plate has been pre-coated with Bovine HSPB1 antibody
Bovine LGMN (Legumain) ELISA Kit Packaging:96 wells The assay plate has beenThe Bovine (LGMN) Legumain ELISA Kit measures Legumain in Bovine samples. The plate has been pre coated with Bovine LGMN antibody. LGMN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine LGMN Antibody is added and binds to LGMN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated LGMN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
