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The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 27 nm
And then biotinylated Bovine CHI3L1 Antibody is added and binds to CHI3L1 in the sample
And then biotinylated Human SEMA3E Antibody is added and binds to SEMA3E in the sample
only those wells that contain Mouse IL1R1
Substrate solution is then added and color develops in proportion to the amount of Rat GGT1
Human IgD (Immunoglobulin D) ELISA Kit Packaging:96 wells The optical density (OD) isThe Human IgD (Immunoglobulin D) ELISA Kit measures IgD in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IgD antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
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