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The concentration of Rat KRT18 in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL1b
the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue colored product that is directly proportional to the amount of leptin present
The Rat HNF1b (Hepatocyte Nuclear Factor 1 Beta) ELISA Kit is a Sandwich enzyme immunoassay
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat THRa
TSPAN1 (Tetraspanin 1) ELISA Kit (Small Sample) Packaging:48 wells The concentration of Rat KRT18The TSPAN1 (Tetraspanin 1) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human TSPAN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human TSPAN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is
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