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Then a biotinylated detection antibody specific for Porcine IL-1Alpha and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then Streptavidin-HRP is added and binds to the Biotinylated DEFB128 antibody
Then a biotinylated detection antibody specific for Human RETN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
DBN1 present in the sample is added and binds to antibodies coated on the wells
And then biotinylated Mouse FNDC5 Antibody is added and binds to FNDC5 in the sample
Mouse LEP (Leptin) ELISA Kit RCM3 Then a biotinylated detection antibodyThe Mouse (LEP) Leptin ELISA Kit measures Leptin in Mouse samples. The plate has been pre coated with Mouse LEPTIN antibody. LEPTIN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse LEPTIN Antibody is added and binds to LEPTIN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated LEPTIN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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