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a horseradish peroxidase conjugated mouse anti-Human IL-10 Antibody is added to the wells and binds to IL-10 in the sample
And then biotinylated Bovine ANGPTL4 Antibody is added and binds to ANGPTL4 in the sample
it binds with PERK bound to precoated antibody
Calculate the concentration of PGA3 in the sample by plotting standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated FGF4 antibody
Human USP11 (Ubiquitin Specific Peptidase 11) ELISA Kit Packaging:96 wells a horseradish peroxidase conjugated mouseThe Human USP11 (Ubiquitin Specific Peptidase 11) ELISA Kit measures USP11 in Human Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti USP11 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
