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Then a biotinylated detection antibody specific for Rat IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
And then biotinylated Equine IL4 Antibody is added and binds to IL4 in the sample
You can calculate the concentration of Mouse LIF in the samples by comparing the OD of the samples to the standard curve
converting the color from blue to yellow
And then biotinylated Human CLDN10 Antibody is added and binds to CLDN10 in the sample
Mouse IL-1A (Interleukin 1Α) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Mouse (IL 1A) Interleukin 1 ELISA Kit measures Interleukin 1alpha in Mouse samples. The plate has been pre coated with Mouse IL1A antibody. IL1A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse IL1A Antibody is added and binds to IL1A in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IL1A antibody. After incubation unbound Streptavidin HRP is washed away during a