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The concentration of Mouse IPF in the samples is then determined by comparing the OD of the samples to the standard curve
The OD value is proportional to the concentration of Human CGRP1
The OD value is proportional to the concentration of Human MMP-3
The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for leptin is immobilized onto the microplate and another monoclonal antibody specific for a different epitope of leptin is conjugated to biotin (biotin conjugate)
Then a biotinylated detection antibody specific for Human IL-12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
NLRC4 (NLR Family, CARD Domain Containing Protein 4) ELISA Kit (Small Sample) Packaging:96 wells The concentration of Mouse IPFThe NLRC4 (NLR Family, CARD Domain Containing Protein 4) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human NLRC4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human NLRC4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB
