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Substrate solution is then added and color develops in proportion to the amount of Rat Shank3
Then TMB substrate solution is added to each well
Substrate solution is then added and color develops in proportion to the amount of Mouse CIRBP
You can calculate the concentration of Human EPO in the samples by comparing the OD of the samples to the standard curve
And then biotinylated Human APH1A Antibody is added and binds to APH1A in the sample
Human BECN1 (Beclin-1) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human BECN1 (Beclin 1) ELISA Kit measures BECN1 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti BECN1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
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