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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CD200
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACTa2
And then biotinylated Human CYP27A1 Antibody is added and binds to CYP27A1 in the sample
Then a biotinylated detection antibody specific for Rat CTXI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Human NGAL in the samples is then determined by comparing the OD of the samples to the standard curve
Human CLDN17 (Claudin 17) ELISA Kit Packaging:96 wells The microtiter plate provided inThe Human (CLDN17) Claudin 17 ELISA Kit measures Claudin 17 in samples. The plate has been pre coated with Human CLDN17 antibody. CLDN17 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CLDN17 Antibody is added and binds to CLDN17 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CLDN17 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
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