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Then a biotinylated detection antibody specific for Rat CASP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
And then biotinylated Mouse DCN Antibody is added and binds to DCN in the sample
it binds with ADAM12 bound to precoated antibody
The OD value is proportional to the concentration of Rabbit C4BP
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat APOB100
Human SOD3 (Superoxide Dismutase 3, Extracellular) ELISA Kit Packaging:48 wells Then a biotinylated detection antibodyThe Human SOD3 (Superoxide Dismutase 3, Extracellular) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Human SOD3. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to Human SOD3. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate
