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Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of E2 in the sample
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TAF12
Only those wells that contain Mouse IgG1
Then Streptavidin-HRP is added and binds to the Biotinylated SIRT1 antibody
Substrate solution is then added and color develops in proportion to the amount of Human LCT
Human 8-iso-PGF2Alpha (8-isoprostane) ELISA Kit Packaging:96 wells Compare OD450 value of sampleThe Human 8 iso PGF2Alpha (8 isoprostane) ELISA Kit measures 8 iso PGF2Alpha in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with 8 iso PGF2. 8 iso PGF2 in the sample or standard competes with a fixed amount of biotin labeled 8 iso PGF2 for sites on a pre coated antibody specific to 8 iso
