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then added a HRP-conjugated antibody specific to Human MIg
Universal 4-HNE in the sample or standard competes with a fixed amount of Universal 4-HNE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 4-HNE
Recombinant Human Asprosin is produced by our Mammalian expression system and the target gene encoding Ser2732-His2871 is expressed with a 8His tag at the N-terminus
Free components are washed away
The concentration of Rabbit BMP4 in the samples is then determined by comparing the OD of the samples to the standard curve
CRP (C-Reactive Protein) ELISA Kit (Mini-Sample) TSG-14 then added a HRP-conjugated antibodyThis CRP (C Reactive Protein) ELISA Kit (Mini Sample) uses the sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to CRP . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CRP and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and