Rat IL33 (Interleukin 33) ELISA Kit (Small Sample) HPSE1 The concentration of Goat EGF

The concentration of Goat EGF in the samples is then determined by comparing the OD of the samples to the standard curve

The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 40 nm

then added a HRP-conjugated antibody specific to Human EFNA1

only those wells that contain Human MPIF1

only those wells that contain Mouse KIBRA

Rat IL33 (Interleukin 33) ELISA Kit (Small Sample) HPSE1 The concentration of Goat EGFThe Rat IL33 (Interleukin 33) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat IL33. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat IL33. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added,