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Substrate solution is then added and color develops in proportion to the amount of Human HCN2
And then biotinylated Human Raptor Antibody is added and binds to Raptor in the sample
You can calculate the concentration of Human TARC in the samples by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Mouse NE/ELA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
CTNNA1 present in the sample is added and binds to antibodies coated on the wells
Human CTSD (Cathepsin D) ELISA Kit TNFR1 Substrate solution is then addedThe Human CTSD (Cathepsin D) ELISA Kit measures CTSD in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CTSD antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
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