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The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TSP-4
Then a biotinylated detection antibody specific for Human KISS1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Human LCN1 in the samples is then determined by comparing the OD of the samples to the standard curve
When the sample containing CD50 is added to the plate
only those wells that contain Mouse INa
Human APAF-1 (Apoptosis Protease Activating Factor-1) ELISA Kit Packaging:96 wells The micro ELISA plate providedThe Human (APAF 1) Apoptosis Protease Activating Factor 1 ELISA Kit measures apoptosis protease activating factor 1 in samples. The plate has been pre coated with Human Apaf 1 antibody. Apaf 1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human Apaf 1 Antibody is added and binds to Apaf 1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated Apaf 1 antibody. After incubation
