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The Mouse SEPP1 (Selenoprotein P) ELISA kit uses the Sandwich-ELISA principle
OC90 present in the sample is added and binds to antibodies coated on the wells
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat RLN
Then a biotinylated detection antibody specific for Human SEMA4D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
NEUROD2 present in the sample is added and binds to antibodies coated on the wells
Human PKP4 (Plakophilin 4) ELISA Kit Matrilysin The Mouse SEPP1 (Selenoprotein P)The Human (PKP4) Plakophilin 4 ELISA Kit measures Plakophilin 4 in samples. The plate has been pre coated with Human PKP4 antibody. PKP4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PKP4 Antibody is added and binds to PKP4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PKP4 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
