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Then Streptavidin-HRP is added and binds to the Biotinylated GSK3B antibody
And then biotinylated Human ANO6 Antibody is added and binds to ANO6 in the sample
Universal LTB4 in the sample or standard competes with a fixed amount of Universal LTB4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal LTB4
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CXCL16
Substrate solution is then added and color develops in proportion to the amount of Human YY1
Human IL-40 (Interleukin 40) QuickTest ELISA Kit Pepsi Then Streptavidin-HRP is added andThe Human IL 40 (Interleukin 40) QuickTest ELISA Kit measures IL 40 in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples into
