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The Human CCL4/MIP1b ELISA kit is a sandwich enzyme immunoassay
The concentration of Guinea pig IL1b in the samples is then determined by comparing the OD of the samples to the standard curve
a horseradish peroxidase conjugated mouse anti-Rat LILRA5 Antibody is added to the wells and binds to LILRA5 in the sample
You can calculate the concentration of Human sIgA in the samples by comparing the OD of the samples to the standard curve
The substrate solution is added to eachwell
CCL14 (Chemokine C-C-Motif Ligand 14) ELISA Kit (Small Sample) Packaging:96 wells The Human CCL4/MIP1b ELISA kitThe CCL14 (Chemokine C C Motif Ligand 14) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human CCL14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human CCL14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate
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