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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LTb
The concentration of Human IgG1 in tested samples can be calculated by comparing the OD of the samples to the standard curve
only those wells that contain Rabbit ADP
Then Streptavidin-HRP is added and binds to the Biotinylated ITSN1 antibody
Substrate solution is then added and color develops in proportion to the amount of Human POMC
Human GATA3 (GATA Binding Protein 3) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human GATA3 (GATA Binding Protein 3) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human GATA3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GATA3 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each
